SWAP-70^−/− DCs do not induce proper expression of RhoB. (A) Expression of SWAP-70, RhoA, and RhoB. CD11c⁺ BMDCs were lysed at different time points after LPS treatment and proteins were resolved by SDS-PAGE and analyzed by Western blotting. Membranes were probed with RhoA and RhoB antibodies. Detection of SWAP-70 and GAPDH were used as control. (B) Panels showing the density values of the relative expression of RhoA and RhoB when compared with expression of GAPDH. Representative data of 2 independent experiments are shown. □ indicates wt; ■, SWAP-70^−/− as indicated. (C) CD11c⁺ BMDCs were lysed at the indicated time periods after LPS treatment and the RhoGTPases activities were investigated. The amount of total RhoGTPases was adjusted to be the same. Representative data of 2 independent experiments are shown. (D) Relative activity of RhoGTPases at the indicated time periods after LPS treatment. Active RhoGTPases were compared with total amounts. Representative data of 2 independent experiments are shown. □ indicates wt; ■, SWAP-70^−/−. *P < .001.