In sublethally irradiated recipients, only MPPs display robust myeloid potential. Sublethally irradiated recipient C57BL/6 mice were transplanted with 5000 MPP (left column), 5000 CLP (middle column), or 5000 DN1 cells (right column) from GFP-transgenic mice. After 5, 7, or 14 days, the bone marrow (A), spleen (B), and thymus (C) of each mouse was analyzed for GFP⁺ donor cells by flow cytometry. Each column represents the level of GFP⁺ chimerism in that tissue, and the different patterns indicate the chimerism of the different lineages. The lineages were determined as follows: Myeloid cells (GM) were defined as Mac-1⁺Gr-1⁺CD11c⁻; dendritic cells (DC) were defined as CD11c⁺; B cells were defined as CD19⁺. T lineage cells were defined as either CD4⁺ (pre-T, CD4⁺) or CD25⁺ (pre-T, CD25⁺) in the thymus. At day 14, most thymocytes were immature, and antibodies to CD4 and CD25 labeled nearly all T lineage cells. The tables below each graph show the frequency (cells/million live cells) of each donor cell lineage for each tissue analyzed and represent the average from 3 transplanted mice. This experiment was performed twice, with similar results.