Dissection of HLA-A2/minor H peptide tetramer staining profiles. (A,C) Definition of tetramer^bright and tetramer^dim gate settings (as indicated by dotted lines) using a tetramer^bright staining cytolytic HY (A) or HA-1 (C) specific T-cell clone titrated into CD8-enriched PBMCs. (B) Analysis of tetramer-binding profiles of 2 female donors (♀9 and ♀6) with male offspring after nonstringent sorting of HY^A2 tetramer⁺ CD8⁺ cells. (D) Analysis of HA-1^A2 tetramer-binding profiles of 2 HA-1^R/R male donors (♂1 and ♂2) with a HA-1^H/R mother after nonstringent sorting of HA-1^A2 tetramer⁺ CD8⁺ cells. The solid box represents the total CD8⁺ tetramer⁺ population; tetramer^bright staining T cells are plotted in black; tetramer^dim staining T cells are plotted in gray. Percentages indicate the distribution of each T-cell subset in the total tetramer⁺ fraction.