Histone H4 acetylation and NF-κB/p65 and STAT1 binding at IRF1 promoter inex vivounstimulated PBMCs. (A) Basal histone H4 acetylation at IRF1 locus was assessed in the ex vivo PBMCs from HIV-S (▵, n = 10) and HIV-R (□, n = 18) individuals using ChIP assay and analyzed with quantitative PCR. (B) NF-κB/p65 binding to IRF1 promoter in ex vivo PBMCs of HIV-S (▵, n = 10) and HIV-R (□, n = 11) participants was examined using ChIP and analyzed with quantitative PCR. Basal level of STAT1 binding to IRF1 promoter in the ex vivo, unstimulated PBMCs of HIV-S (▵, n = 10) and HIV-R (□, n = 18) individuals was also examined. Bars represent mean values. *P < .05. Not significant (P > .05).