Stimulation of TRAT-specific T cells with human cells of different lymphoid lineages. HLA Cw*0602⁺ and DRB*0401⁺ fresh PBMCs, monocyte-depleted PBMCs, B-cell blast, EBV-infected B-cell line, monocytes, and GM-CSF and IFN-γ–treated monocytes were presensitized with TRAT peptide or left uncoated and then exposed to TRAT-specific T cells. After incubation, these T cells were analyzed for IFN-γ expression by the use of ICS assays.