Phenotype of monocyte-derived mDCs generated under hypoxic conditions. Human monocytes were cultured for 48 hours with IL-4 and GM-CSF followed by incubation with the proinflammatory mediators, TNF-α, IL-1β, IL-6, and prostaglandin E2 for an additional 48 hours under 20% O2 (mDCs) or 1% O2 (H-mDCs) conditions, and expression of the indicated cell surface molecules was analyzed by flow cytometry after 4-day culture, as described in “Flow cytometry.” The expression profile of unstimulated monocytes is shown for comparison. Cells were gated according to their light scatter properties to exclude cell debris. Solid histograms represent the fluorescent profile of cells stained with specific FITC-conjugated Abs; and open histograms, the fluorescent profile of cells stained with isotype-matched controls. Data are plotted as fluorescence intensity on a log scale versus the number of positive cells. In each histogram, the percentage of positive cells is indicated. Results are from one representative experiment of 10 performed with cells from different donors.
