Effects of acute hypoxia on TREM-1 expression. Four-day mDCs were exposed to hypoxia for 24 hours, and (A) TREM-1 and CAXII mRNA expression was assessed by quantitative RT-PCR. Data from one representative experiment of 3 performed are expressed as in the legend to Figure 3A. (B) TREM-1 surface expression was evaluated by flow cytometry. Solid histograms represent the fluorescent profile of TREM-1-expressing cells; and open histograms, the fluorescent profile of cells stained with the isotype-matched control Ab. The percentage of TREM-1⁺ cells and the mean fluorescent intensity are indicated. Results from one of 3 independently tested donors are shown.