Figure 4
GATA-1, p53TAD, and a GATA-1 DNA-binding site do not form a ternary complex. (A) Overlay of the 2D 1H-15N HSQC spectra for a 0.5 mM sample of 15N-labeled GATA-1 L plus CF/DNA complex (black) and in the presence of 0.5 mM unlabeled p53TAD (red). (B) Overlay of the 2D 1H-15N HSQC spectra for a 0.5 mM sample of 15N-labeled p53TAD in the free form (black) and in the presence of 0.5 mM unlabeled GATA-1 L plus CF/DNA complex (red).
