Ex vivo deletion of survivin in mouse bone marrow cells leads to increased polyploidization of megakaryocytes. Lineage-depleted bone marrow cells from Sur^fl/fl mice were infected with MSCV-GFP or MSCV-Cre-GFP and cultured with TPO (20 ng/mL) for 3 days. Cells were stained with PE-labeled anti-CD41 and DAPI and analyzed by flow cytometry. CD41 expression (A) and DNA content (B) of the GFP⁺ population are shown. Representative flow and bar graphs depicting the average ± SEM for 5 to 6 mice per group are shown. *P = .003; **P = .001 (C). Deletion of the floxed region was monitored by multiplex PCR. PCR of DNA extracted from GFP⁺ sorted MSCV-GFP or MSCV-Cre-GFP transduced cells is shown. Percentage excision (average of 2 experiments) is indicated below the blot.