Allogeneic BM engraftment in AKR/J → BALB.K (H2^k) mice. Recipient BALB.K mice were treated with TBI 300 or 400 cGy and injected with BM or HSCs from MHC-matched AKR/J donors. (A) Percentage of mice with donor engraftment 6 weeks after transplantation. For engrafted mice, blood chimerism at indicated time points are shown with (B) representative FACS analysis for T cells using Thy1.1 (donor) and Thy1.2 (host) cell-surface markers, (C) representative PCR for B-cell and myeloid chimerism using DNA extracted from FACS-sorted B220⁺ and Mac1/Gr1⁺ blood cells amplified for the DNA marker D6Mit3, and (D) dot plot summarizing T-cell chimerism.