Allogeneic BM engraftment in AKR/J → B10.BR (H2k) mice. Recipient mice were given sublethal TBI, alone or with anti-CD4 (GK1.5) and/or anti-CD8 (53-6.7), before infusion of donor BM or HSCs. Shown are the percentages of mice with donor engraftment and chimerism characteristics at 6 weeks after transplantation. T-cell chimerism was evaluated by FACS for Thy1 surface markers. B-cell and myeloid chimerism was evaluated by PCR for D8Mit224 DNA markers of FACS-sorted B220+ and Mac1/Gr1+ blood cells.