Correlation between the presence of mutations in the BCL2 gene and BCL2 protein expression by immunohistochemistry using clones 124 and E17. (A) BCL2 protein expression by immunohistochemistry and corresponding amino acid sequence derived from sequencing the BCL2 gene in 3 BCL2⁺/MYC⁺ lymphoma samples. The affected amino acid changes are highlighted in red. (B) DLBCL sample (sample 2) stained with the BCL2 antibody clone 124 (Dako North America; original magnification ×200). (C) The same DLBCL sample (sample 2) stained with the BCL2 antibody E17 (Epitomics; original magnification, ×200). Ref indicates reference genome hg 19, May 2009; IHC, immunohistochemistry; P, positive; and N, negative.