Dual target specificity of CRA. (A) BM-DCs (from BALB/c mice) and/or CD3⁺ T cells (from C57BL/6 mice) were preincubated for 24 hours with CRA (10 μM) or vehicle alone. After extensive wash, the DCs and T cells were then cocultured in the presence or absence of CRA (10 μM). Data shown are ³H-thymidine uptake on day 4 (mean ± SD, n = 3). (B) BM-DCs (from BALB/c mice) were preincubated for 24 hours with CRA (10 μM) or vehicle alone, washed extensively, and then cocultured with T cells (from C57BL/6 mice). Data shown are ³H-thymidine uptake on day 4 (mean ± SD, n = 3). Asterisks indicate statistically significant (*P < .05; **P < .01) differences compared with the control panel in which DCs and T cells were both pretreated with vehicle alone.