Anti–AAV-1 antibody subclass analysis. Serum levels of IgG1, IgG2, IgG3, IgG4, and IgM (average ± SD, ng/mL). ♦ indicates subjects with positive T-cell response to the AAV capsid measured by ELISpot (subjects A, D-E, and H); , subjects with no detectable T-cell response to the AAV capsid measured by ELISpot (subjects B-C, F-G); *P < .05.