Migration of APCs. (A) Donor BALB/c APC subsets were labeled with CFSE and injected IV into B6 mice (n = 4); 24 hours later, migration of APCs to various organs and tissues was determined by detecting CFSE⁺ cells using flow cytometric analysis. (B) Comparison of homing of cDCs and pDCs (n = 4). The horizontal dashed line indicates the percentage of cDCs in the input sample. (C-E) Immunostaining of frozen sections of the thymus injected with CMRA- or CFSE-labeled FLDCs. Frozen sections of thymus were stained with Cy5–anti-CD8 (53-6.7) mAb, the lectin UEA-1, biotin–anti-CD11c (N418) mAb, and PE–anti-B220 mAb, respectively. Scale bars represent 200μm (C left) or 20μm (C right, D-E). Data in all panels are representative of 3 independent experiments.