Splenectomy reduces the development of marrow fibrosis and new bone formation in Gata1^low/+ mice. (A) Hematoxylin-eosin, (B) Gomory-silver, and (C) Mallory staining of long bones from Gata1^low/+ females at different time points after splenectomy, as indicated. Results are compared with those of untreated Gata1^low/+ female littermates at 9 months of age. Results are representative of those obtained in multiple experiments. Representative MKs and bony trabeculae are indicated by arrows and arrowheads in the insets, respectively. Magnification is ×20 in the panels and ×40 in the insets. (D) Levels of Mallory staining of the femur of untreated and splenectomized Gata1^low/+ mice. Results are presented as mean ± SD determinations of at least 3 mice per experimental group and are compared with those observed in untreated wild-type mice of comparable age (shaded area). The levels of Mallory staining were determined by analyzing with the MetaMorph program at least 3 randomly chosen bone areas per mouse. Values statistically different (P < .05 and P < .01) from those observed in untreated wild-type and Gata1^low/+ females are indicated by § and *, respectively.