Figure 4
Figure 4. Induction of in vitro angiogenesis. Endothelial cell growth in the AngioKit model. (A) Box plot summarizing the ELISA results. Unlike those of MBC supernatants of BMPCs, pMMCs and HMCLs show a significant induction of tubule formation compared with medium control. *Significant difference compared with medium control (P < .05). (B) Immunostaining with monoclonal anti-human CD31 antibody: medium control (RPMI 1640), positive control (VEGF), negative control (suramin) as well as supernatants of memory B-cell samples (MBC1-3), normal bone marrow plasma cell samples (BMPC1-3), primary myeloma cell samples (pMMC1-3), and the myeloma cell lines SKMM-2, XG-10, and U266. Original magnifications ×40.

Induction of in vitro angiogenesis. Endothelial cell growth in the AngioKit model. (A) Box plot summarizing the ELISA results. Unlike those of MBC supernatants of BMPCs, pMMCs and HMCLs show a significant induction of tubule formation compared with medium control. *Significant difference compared with medium control (P < .05). (B) Immunostaining with monoclonal anti-human CD31 antibody: medium control (RPMI 1640), positive control (VEGF), negative control (suramin) as well as supernatants of memory B-cell samples (MBC1-3), normal bone marrow plasma cell samples (BMPC1-3), primary myeloma cell samples (pMMC1-3), and the myeloma cell lines SKMM-2, XG-10, and U266. Original magnifications ×40.

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