Twsg1 mRNA expression in murine thalassemia. Murine Twsg1 mRNA in (A) spleen, (B) liver, and (C) bone marrow from wild-type mice (WT, n = 7), Hbb^th3/+ β-thalassemia intermedia mice (th3/+, n = 13), and Hbb^th3/th3 β-thalassemia major mice (th3/th3, n = 5) was determined by quantitative PCR using total RNA isolated from the tissues. Bars represent mean values; *P < .05 compared with wild-type. (D) Murine Twsg1 protein (Twsg1) expression in spleen from wild-type mice (WT), Hbb^th3/+ β-thalassemia intermedia mice (th3/+), and Hbb^th3/th3 β-thalassemia major mice (th3/th3) was detected by immunoblotting using a rabbit anti–serum-specific Twsg1. Anti–β-actin antibody were used as internal control. Splenic tissues from 2 mice were studied for comparison (#1, #2).