Peripheral T cells in ΔSAM–SLP-76 knockin mice. Splenocytes and peripheral lymph node cells from 5-week-old SLP-76^m/+ and SLP-76^m/m littermates were analyzed by flow cytometry. (A) Total numbers of splenocytes, lymph node cells, and CD4⁺ and CD8⁺ T cells. Five mice for each genotype were analyzed. Statistical analysis was performed using 2-tailed Student t test. *P < .05; **P < .01. (B) The percentages of TCR-β⁺ cells in spleens and lymph nodes. (C) Expression of CD4 versus CD8 on splenocytes and lymph node cells. (D) Expression of CD44 versus CD62L on CD4⁺ gated cells in spleens and lymph nodes. (E) Expression of CD25 versus CD69 on CD4⁺ gated cells in spleens. FACS plots shown are from 1 representative of 5 mice analyzed. The numbers represent the average percentages of the gated populations.