Boolean gating of NK cell subsets expressing discrete combinations of inhibitory KIRs and NKG2A. Gating scheme to identify subsets of CD56^dim NK cells expressing single inhibitory KIR and combinations thereof with or without coexpression of NKG2A. Doublet cells and CD14⁺ cells were excluded based on an FSC-area versus FSC–height gate and CD14 expression, respectively. Gates were set on live CD3⁻ cells as determined by staining with a dead cell marker (DCM) and anti-CD3.