Figure 2
![Figure 2. CalDAG-GEFI and P2Y12 synergize in Cvx-dependent activation of Rap1 and ERK-MAPK. Washed WT or CalDAG-GEFI−/− (knockout [KO]) platelets were stimulated in standard aggregometry with (A) 100 ng/mL (low dose [LD]) or (B-C) 500 ng/mL (high dose [HD]) Cvx. Platelets were activated in the presence (C) of 75 μM 2-MesAMP to inhibit signaling by P2Y12. Rap1-GTP and phospho-ERK were determined in samples withdrawn at the indicated time points. Total ERK1/2 levels (bottom panel) were determined as a loading control. Results are representative of 3 individual experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/12/10.1182_blood-2009-04-218768/4/zh89990941970002.jpeg?Expires=1735597286&Signature=I3rx-n81zxAqWG0WzF1WtCs04-Er8nGqb2Y7a5hioD4W3TJEfvQj5b3FPps1nBnFcQf3DpS4NQT~dQKPpDtjRFIf7h6r5N44yBUU83s6YIcaC4fp-xka3LLovuWgA5hFxhisN2rula59OpHGAaVAEbPmhLq~GV-ZfzpsYF0VMn--7LycmtzxKz3zyRowH6JT0GCwS5Aj5fOKvHPo3LvNA1b5VEczoBvdBtnTSq2aPnjFVmFio~NIw5VDSVaenZFR3xb6D4c478ibyUMfAHTSBZlIC52JDQWGps9b4YXAf6wqib9WaGUAmN2LHxpJs6iTlPzZRxIICMqYbajYwSuMtA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CalDAG-GEFI and P2Y12 synergize in Cvx-dependent activation of Rap1 and ERK-MAPK. Washed WT or CalDAG-GEFI−/− (knockout [KO]) platelets were stimulated in standard aggregometry with (A) 100 ng/mL (low dose [LD]) or (B-C) 500 ng/mL (high dose [HD]) Cvx. Platelets were activated in the presence (C) of 75 μM 2-MesAMP to inhibit signaling by P2Y12. Rap1-GTP and phospho-ERK were determined in samples withdrawn at the indicated time points. Total ERK1/2 levels (bottom panel) were determined as a loading control. Results are representative of 3 individual experiments.
