Figure 6
![Figure 6. Exogenous TxA2 induces firm adhesion of CalDAG-GEFI−/− platelets to collagen. Whole blood from WT or CalDAG-GEFI−/− mice was perfused over a collagen surface at venous shear rates (400 seconds−1). (A) Representative images showing platelets firmly adherent to the collagen surface. U46619 (2 μM) was added to CalDAG-GEFI−/− (knockout [KO]) whole blood immediately before the perfusion. A total of 100 μM 2-MesAMP was added 10 minutes before the perfusion. Scale bar = 10 μm. (B) Quantification of adherent platelets. The data are presented as the mean ± SEM of the absolute number of platelets per 0.02 mm2 surface area. n = 9; 3 different blood preparations. **P < .01.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/12/10.1182_blood-2009-04-218768/4/zh89990941970006.jpeg?Expires=1735598123&Signature=dE-U93W7ytIlWp7D8fLJANRxfKxjg87Lw~nSgoCVzmfxwRRsYFRYEujwgmPHTtQ8gUVBAOSkuHeWsMUklVw~zw5Mq-cw86kYkMfDqrxlJ~LkGxcRmRFNJ6cIJK8XfWsBvJKwNExR2mkkZGY55ypKkb7hStMujGvPyHkYO-39aOPBkCDP3TN4m4PQIsNOhBiTeiIwtxyZNE-QAToyIT7pxr9tkt80cI54PLKAj3HU52B2cfHjZAInZ~rLzwOjtsgE1Mt-MlBJ8YkX8fKLZrykaMC9tNZe9SrsfEzKYehfBuzXSbNCrFtpE0AliyclnsqN~GG2EdqbPeISZmPGn5Cgcw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Exogenous TxA2 induces firm adhesion of CalDAG-GEFI−/− platelets to collagen. Whole blood from WT or CalDAG-GEFI−/− mice was perfused over a collagen surface at venous shear rates (400 seconds−1). (A) Representative images showing platelets firmly adherent to the collagen surface. U46619 (2 μM) was added to CalDAG-GEFI−/− (knockout [KO]) whole blood immediately before the perfusion. A total of 100 μM 2-MesAMP was added 10 minutes before the perfusion. Scale bar = 10 μm. (B) Quantification of adherent platelets. The data are presented as the mean ± SEM of the absolute number of platelets per 0.02 mm2 surface area. n = 9; 3 different blood preparations. **P < .01.
