Rheb2 overexpression enhanced primary mouse hematopoietic progenitor colony formation and survival. (A) Real-time PCR expression of Rheb2 in FACS-sorted subsets of primary mBM cells, 2 or 3 replicates per cell type. (B) Numbers of CFU-GM and CFU-GEMM colonies produced by Mieg3- and Rheb2-transduced mBM cells (3 combined independent experiments, each done in triplicate; mean ± SEM). *P < .05. (C) Numbers of CFU-GM colonies produced in duplicate expanded liquid cultures (mean ± SD). *P < .05. (D) Survival of Mieg3- and Rheb2-transduced mBM cells in delayed growth factor colony assays, representative of 2 experiments each done in triplicate (mean ± SD).