Rheb2 overexpression impaired total donor cell engraftment but transiently expanded phenotypic stem/progenitor cells in vivo in short-term competitive repopulation experiments. (A) PB chimerism of transduced GFP⁺ donor (CD45.2⁺) cells and total donor (CD45.2⁺) cells in recipient animals (at least 5 animals in each group at each time point; mean ± SEM). (B) Chimerism of absolute numbers of transduced GFP⁺ donor cells (CD45.2⁺) in the BM of recipients (at least 5 animals in each group at each time point; mean ± SEM). (C) Representative FACS plots showing the percentage of GFP⁺ cells within the immature LS population in recipient animals at 2 weeks after transplantation. (D) The absolute numbers of GFP⁺LS cells in the BM of recipients (at least 5 animals in each group at each time point; mean ± SEM). *P < .05. **P < .01. NS indicates not significant.