Shear-dependent membrane tether formation. (A-B) DiIC₁₂-labeled transgenic platelets were perfused over human VWF at a rate of 1800 s⁻¹ followed by an increase in shear rate to 10 000, 20 000, or 40 000 s⁻¹. Metamorph stacks (500 frames) were acquired at approximately 3 fields per flow, and 5 fields per flow were analyzed for total length of membrane deposited and number of tethers deposited on the VWF matrix as mean (± SEM) for n = 4-5 independent experiments, *P < .05). (C) Typical fields are shown at a rate of 20 000 and 40 000 s⁻¹ to demonstrate the significant difference between hGPIbα^FW and hGPIbα^WT (also shown in supplemental Video 1).