Defective thrombus formation and prolonged bleeding times in CLEC-2–deficient mice. Mice received vehicle or INU1 (8 μg/g) and were analyzed after 5 days. (A-C) Mesenteric arterioles were injured with FeCl₃, and adhesion and thrombus formation of fluorescently labeled platelets were monitored in vivo by fluorescence microscopy. (A) Time to appearance of first thrombus larger than 10 μm and (B) time to vessel occlusion are shown. Each symbol represents 1 individual. (C) Representative images are depicted. Bar represents 50 μm. Each symbol represents 1 individual. * indicates occlusion of the vessel. (D) Tail bleeding times in control and CLEC-2–deficient mice. Each symbol represents 1 individual.