Figure 2
Figure 2. A20 gene inactivation in the genomic subgroups of DLBCL and MCL. (A) Differential A20 expression in the genomic subgroups of DLBCL and MCL. GCB and ABC DLBCL were divided into subgroups with heterozygous A20 deletion and without A20 deletion. (Top panel) Data from DNA microarray analysis of A20 and NF-κB target gene expression in DLBCL clinical samples. Multiples of relative expression are shown according to the color scale. A20+/− indicates the cases in the heterozygous A20 deletion subgroup; A20+/+, the cases in the subgroup without A20 deletion. The NF-κB target gene set used in this study corresponds to Davis et al.19 The box-and-whisker diagrams depicted in the bottom panel represent median expression values and the interquartile range of A20 in the GCB and ABC DLBCL subgroups. Scale bar represents 5 percentile ranges of A20 expression. (B) Real-time RT-PCR analysis of A20 expression in DLBCL and MCL cases. Among ABC DLBCL cases, 14 patients with A20 loss and 14 patients without A20 loss were analyzed, and analysis of 7 patients with A20 loss and 11 patients without A20 loss was performed among MCL cases. Expressions were normalized on the basis of the corresponding β-actin content, and relative expression levels were established by comparing them with those of healthy male activated B-cell control. *Cases with A20 promoter methylation. (C) Correlation between promoter methylation and expression of A20 in DLBCL and MCL. A20 promoter methylation was subjected to MSP analysis. The location of the CpG and MSP primer regions analyzed in this study is shown in the top panel. (Bottom panel) The inverse correlation between A20 promoter methylation and gene expression analyzed by MSP. A20+/− indicates the cases in the heterozygous A20 deletion subgroup; A20+/+, those in the subgroup without A20 deletion. (D) A20 promoter methylation status in DLBCL and MCL tissues. The A20 gene promoter was methylated in colorectal cancer tissues. M denotes the PCR products of the amplified methylated DNA; and U, those of the unmethylated DNA.

A20 gene inactivation in the genomic subgroups of DLBCL and MCL. (A) Differential A20 expression in the genomic subgroups of DLBCL and MCL. GCB and ABC DLBCL were divided into subgroups with heterozygous A20 deletion and without A20 deletion. (Top panel) Data from DNA microarray analysis of A20 and NF-κB target gene expression in DLBCL clinical samples. Multiples of relative expression are shown according to the color scale. A20+/− indicates the cases in the heterozygous A20 deletion subgroup; A20+/+, the cases in the subgroup without A20 deletion. The NF-κB target gene set used in this study corresponds to Davis et al.19  The box-and-whisker diagrams depicted in the bottom panel represent median expression values and the interquartile range of A20 in the GCB and ABC DLBCL subgroups. Scale bar represents 5 percentile ranges of A20 expression. (B) Real-time RT-PCR analysis of A20 expression in DLBCL and MCL cases. Among ABC DLBCL cases, 14 patients with A20 loss and 14 patients without A20 loss were analyzed, and analysis of 7 patients with A20 loss and 11 patients without A20 loss was performed among MCL cases. Expressions were normalized on the basis of the corresponding β-actin content, and relative expression levels were established by comparing them with those of healthy male activated B-cell control. *Cases with A20 promoter methylation. (C) Correlation between promoter methylation and expression of A20 in DLBCL and MCL. A20 promoter methylation was subjected to MSP analysis. The location of the CpG and MSP primer regions analyzed in this study is shown in the top panel. (Bottom panel) The inverse correlation between A20 promoter methylation and gene expression analyzed by MSP. A20+/− indicates the cases in the heterozygous A20 deletion subgroup; A20+/+, those in the subgroup without A20 deletion. (D) A20 promoter methylation status in DLBCL and MCL tissues. The A20 gene promoter was methylated in colorectal cancer tissues. M denotes the PCR products of the amplified methylated DNA; and U, those of the unmethylated DNA.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal