MSI2 staining intensities. Representative images are shown for 1+, 2+, and 3+ MSI2 staining in the nuclear and cytoplasmic compartments. Specifically, 1+ intensity is shown for nuclear (A) and cytoplasmic (B) staining, 2+ intensity for nuclear (C) and cytoplasmic (D) staining, and 3+ intensity for nuclear (E) and cytoplasmic (F) staining. (A-F) Original magnification ×400; sections were stained with diaminobenzidine and H&E. Manual analysis derived H-scores for nuclear and cytoplasmic MSI2 staining (as detailed in “Methods”) and Aperio image analysis measured nuclear and total cellular (combined nuclear and cytoplasmic) staining, using the illustrated intensity cut-offs. Micrographs were imaged using a Nikon Eclipse 80i microscope, with a Nikon Plan Apo 40×/0.95 air objective and captured using a Nikon DS-F digital camera with NIS-Elements D 3.1 software, with manipulation in CorelDRAW X3.