Frequency distributions for manual and Aperio data. (A-B) Frequency distributions for manually measured cytoplasmic (A) and nuclear (B) H-scores. The number of cases present with H-scores in each interval are shown by bars, using H-score intervals of 25 for cytoplasmic and 20 for nuclear H-scores. More than 50% of cases had cytoplasmic H-scores < 50, and > 50% of cases had nuclear H-scores < 20. (C) Scatter plot showing relationship between manually measured cytoplasmic and nuclear H-scores demonstrating high degree of correlation between the cytoplasmic and nuclear H-scores (R² = 0.898). (D-E) Frequency distributions for Aperio measured percentage of cells showing total cellular (D) and nuclear (E) positivity. The number of cases present with percentage positivities in each interval are shown by bars, using percentage intervals of 5 for both total cellular and nuclear percentages. More than 50% of cases had total cellular MSI2 positivity < 5%, and > 50% of cases had nuclear positivity < 10%. (F) Scatter plot showing the relationship between Aperio measured total cellular and total nuclear percentage positivity, demonstrating high degree of correlation between the cellular and nuclear percentage positivities (R² = 0.8000). (G) Scatter plot showing the relationship between manually measured H-scores (combined cytoplasmic and nuclear scores) and Aperio measured H-scores, demonstrating high degree of correlation between the manually and Aperio measured H-scores (R² = 0.8795).