Genes aberrantly methylated in MDS contain common motifs for DNA binding proteins. Motif analysis with the FIRE algorithm showed a significant overrepresentation of several DNA motifs in promoter regions differentially methylated between MDS and normal CD34⁺ controls. The yellow color in the heatmap reflects overrepresentation of a motif in a given group, and the blue color represents underrepresentation of the motif. For each motif, we also specify (1) location of the motif; (2) statistical significance of the motif expressed as z scores; (3) robustness of the findings, ranging from 0 of 10 to 10 of 10; (4) conservation index; (5) seed that gave rise to the motif; and (6) name of the closest known motif in our motif database.