The effect of DZNep on colony formation of LSCs from AML patients and normal HSCs. Methycellulose-based colony assay performed in CD34⁺CD38⁻ BM cells form 3 AML patients and 3 healthy donors. Each data point represents the average of 3 samples with triplicate platings normalized to DMSO control. The data were analyzed by the Student t test comparing normal HSCs vs LSCs (*P < .05, **P < .01). All the AML samples were obtained through an approved protocol from the university institutional review board and follow the Helsinki protocol.