Cellular localization of BMP6 in hepatic iron overload. BMP6 expression was detected by immunohistochemistry in (B-C) wild-type B6 mice with secondary iron overload and (D) Hfe-deficient D2 mice. These mice have similar degrees of iron loading. As seen in serial liver sections, whereas iron deposits visualized by Perls staining (A) are predominantly periportal, BMP6 staining is mostly centrilobular (B). Mutant animals and mice with secondary iron overload have intense staining at the basolateral membrane domain of hepatocytes (C-D). Original magnification, ×100 (A-B) or ×400 (C-D). Images were captured using a Leica DMR microscope equipped with an HC PL Fluotar 10×/1 (A-B) or 40×/1 (C-D) numeric aperture objective lens and a Leica DFC 300 Fx camera. They were processed using Leica IM50 image acquisition software.