Analysis of the dynamics of anti and proapoptotic Bcl-2 protein complexes in KMM-1 resistant cells treated by ABT-737. (A) Immunoprecipitation of KMM-1 cell lysates using the indicated antibodies. (B) Bcl-2 immunoprecipitation of KMM-1 cell lysates (300 μg) treated or not with 40nM ABT-737 for both 1 and 6 hours. The immunoprecipitates and the outputs (IP supernatants) were analyzed for the presence for the indicated proteins by immunoblotting. (C) Supernatants of Bcl-2 immunoprecipitation (300 μg) were used for Mcl-1 immunoprecipitation. Immunoblotting was preformed as described. The asterisk (*) corresponds to the immunoglobulin light chain.