In situ hybridization histochemistry of Zfp36l2 mRNA expression at E14.5. Sagittal sections of E14.5 WT and KO embryos were hybridized with Zfp36l2 (A) or GAPDH (B) antisense probes. The specificity of the Zfp36l2 antisense probe was demonstrated by the absence of signal seen with the KO embryo compared with the WT embryo. A sense probe for Zfp36l2 produced essentially no signal at this exposure time (data not shown). Note the weak hybridization seen in the KO placenta in the maternal decidua, a tissue that should be heterozygous for Zfp36l2. (C) Neighboring sections stained with hematoxylin and eosin. b indicates brain; d, decidua; h, heart; l, liver; ys, yolk sac; and p, placenta. Original magnifications ×40, obtained using the Aperio Scanscope T2 Scanner (Aperio Technologies Inc). Scanned images were imported into Aperio Imagescope, Version 6.25.0.1117.