Infection of DC with listeria or pretreatment of DC with TLR agonists enhances cytokine secretion by HJ1 T cells. HJ1 T cells were enriched from the liver of HJ1Tg/hCD1Tg/Rag−/− mice. (A) WT and hCD1Tg BMDC were either left untreated, were pretreated with Pam3Cys or LPS, or were infected with LM before co-culture with HJ1 T cells. After 48 hours, cytokine levels in the supernatant were detected by ELISA. Results are expressed as the mean ± SD. Statistical significance was evaluated by comparing cytokine secretion between HJ1 T-cell cultures stimulated with treated hCD1Tg BMDC and cultures stimulated with untreated hCD1Tg BMDC. (B) HJ1 T cells were cocultured with human monocyte-derived DC in the presence of anti-CD1b or isotype mAb for 48 hours. Cytokine secretions were examined by ELISA. Results are expressed as the mean ± SD. Statistical significance was evaluated by comparing cytokine secretion by HJ1 T-cell cultures in the presence of anti-CD1b with in the presence of isotype Ab. Data are representative of 2 independent experiments. (C) HJ1Tg T cells and Vα14Tg NKT cells were stimulated with Pam3Cys-pulsed WT or hCD1Tg BMDC for 48 hours. Bar graphs depict the mean ± SD for cytokine levels in the supernatant as detected by ELISA. Statistical significance was evaluated by comparing cytokine secretion between HJ1 T-cell cultures stimulated with treated-hCD1Tg BMDC and cultures stimulated with treated-WT BMDC, or by comparing cytokine secretion between HJ1Tg T cells and iNKT cells on stimulation with Pam3cys-treated hCD1Tg BMDC. **P < .01. Data are representative of 3 independent experiments.
