Representative images of Z-series confocal microscopy of kidneys. Transplanted, BMC-derived, GFP-positive cells are seen in green. (A,C,E) F-actin intermediate filament staining by Bodipy-phalloidin (red) and nuclei staining by DAPI (blue). (B,D,F) Same picture without Bodipy-phalloidin to better observe the GFP-positive cells. (A-B) Kidney section of Ctns^−/− mice treated with Ctns^−/− BMC; no GFP-positive cells are observed establishing the threshold for GFP detection. These instrument settings were saved and used for all the subsequent imaging.(C-D) Representative kidney section of WT mice transplanted with GFP BMCs. Few GFP-positive cells are observed. (E-F) Kidney section of Ctns^−/− mice transplanted with GFP BMCs. Abundant GFP-positive BMC-derived cells are evident. Scale bar represents 20 μm (40×/1.3 NA oil objective).