Downstream target identification for KSHV-regulated miRNAs. (A) PCA using differentially expressed genes (P < .005 plus > 1.5-fold change). Each spot represents a single array. (B) Venn diagram showing the principle of selecting putative miRNA-regulated targets. Array data showing overexpression of the miRNA is shown in black, LECs versus KLECs are shown in blue, BECs versus KBECs are shown in green, and the computational prediction is shown in red. The number at the intersection indicates the overlapped genes across all groups. (C) Details of miRNA targets, with 9 common targets being found for miR-221 and miR-222. (D-E) Heat maps and histograms showing that our predicted miRNA targets are still deregulated in KS tissues.