AMH levels and AFC in TM women and normo-ovulatory controls. (A) AMH levels in the thalassemia women, 25 years and older (n = 23, red circles) were compared with normal controls (●; n = 759), showing that the slopes of the regression lines against age were not statistically different (P = .56). The slope was significant for the normal controls (P < .0001; 95% CI, −1.867 to −1.406) and for the thalassemia patients (P < .03; 95% CI, −2.323 to −0.1142), implying an association with age. There was a 5.0pM (95% CI, 13.4 to 26.8) difference between the group means. The levels in the thalassemia women were in the low-normal range of normal and dropped to lower levels in women older than 30 years. (B) Age-dependent AFC in thalassemia women and normal controls. AFC number includes all counted follicles 2-10 mm in size, in thalassemia women, and in the cohort of normal controls (n = 769).