p15INK4B promoter methylation in a complete responder (patient 4). (A) Genomic DNA BSS of 10 clones at 3 different time points (days 0, 15, and 29) during the first cycle of treatment for patient 4. The numbers below the horizontal bar describe the position of the CpGs relative to the transcription start site (+1). Notations as per Figure 2. (B) MSP gel for the DNA from CD34− and enriched CD34+ cells at 3 different time points day 0 (d0), days 15 to 16 (d15), and days 28 to 29 (d29) of therapy. U indicates unmethylated lane; and M, methylated lane. In vitro methylated (IVD) DNA was used as a positive control for methylated status, HCT 116 double knockout (DKO) for DNMT1 and DNMT3b was used as a positive control for unmethylated status, and water (H2O) was used as a negative control for the PCR reaction.
