Treatment with 5AC and entinostat induces transient and reversible global DNA hypomethylation. LINE-1 methylation reversal was monitored by DNA pyrosequencing in 5 patients from different 5AC dose cohorts on days 0, 15, and 29 during the first cycle of treatment. The bar graph demonstrates the average of LINE-1 methylation from the 5 patients at the designated time points. The values represent the mean of duplicate runs ± SD. * indicates significant difference from day 0 at P ≤ .015.