Specific binding of PEG-Liposome to rFVIII by Biacore analysis. (A) A representative Biacore binding profile of the flow-through PEG-Liposome and the negative control POPC-Liposome to rFVIII or HSA immobilized on a CM5 chip at a coating density of 1200 RU. (B) The percentage of difference in the maximal binding of the respective liposome to immobilized rFVIII or HSA. Results presented are mean ± SD from 4 Biacore measurements on separately derivatized CM5 chips. The binding of PEG-Liposome to rFVIII is significantly different from that by POPC-Liposome (P < .001, 2-tailed t test).