rFVIII-PEG-Lip induces more robust clot formation in whole blood ex vivo. (A) Calibration curve of the clotting time in ROTEM versus levels of rFVIII spiked in freshly collected naive HemA mouse blood. Results presented are mean ± SD from 6 individual blood samples for each rFVIII dose. (B-D) ROTEM of whole blood collected from the vena cava of HemA mice at 24 hours after dosing with 8 IU/kg rFVIII or rFVIII-PEG-Lip (n = 20 mice/treatment). Within 5 minutes from collection, 300 μL blood was recalcified and immediately analyzed by ROTEM. Results presented are (B) clotting time, (C) clot formation time, and (D) alpha angle from each individual blood sample. Bar represents the median in each treatment group. Two-tailed P values were generated by Mann-Whitney test.