Effect of IL-2 signaling on differentiation of intratumoral T cells in FL. (A) Representative histograms (n = 3) showing proliferation measured by CFSE staining of T cells treated with IL-2, or anti–IL-2, or anti–IL-2 plus anti–IL-2Rα and anti–IL-2β Abs. Proliferative capacity was expressed by calculating the number of CFSE^dim cells. (B) Representative plots (n = 6) showing the expression of IL-17, IFN-γ, or Foxp3 in CD4⁺ T cells treated with or without IL-2, anti–IL-2, anti–IL-2Rα, or anti–IL-2Rβ Ab. (C) Summary of the numbers of T_H1 (CD4⁺IFN-g⁺) or T_H17 (CD4⁺IL-17⁺) or T_reg (CD4⁺Foxp3⁺) cells induced by IL-2 or anti–IL-2, anti–IL-2Rα, or anti–IL-2Rβ Ab. The induction of T_H1 or T_H17 or T_reg cells was converted to logarithm number. (D) Representative plots (n = 4) showing proliferation measured by CFSE staining of CD8⁺ T cells cocultured CD4⁺ T cells pretreated with or without IL-2. (E) Summary of viability measured by annexin/PI assay of CD4⁺CD25⁻ conventional (T_con) or CD4⁺CD25⁺ regulatory (T_reg) T cells treated with or without IL-2 (n = 3).