Analysis of the HMGB1-mediated THP-1 adhesion and migration in HUVECs. (A) Confluent HUVECs were incubated with HMGB1 (1 μg/mL for 16 hours) after treating cells with indicated concentrations of APC for 3 hours, and the THP-1 adherence to HUVECs was monitored as described in “Methods.” (B) The HMGB1 (1 μg/mL for 16 hours) mediated migration of THP-1 across HUVEC monolayers was analyzed after treating cells with indicated concentrations of APC. (C-D) The same as panels A and B, except that MeizoTh (white bars) or PC-gla/MeizoTh (black bars) were used to treat cells for 3 hours before stimulation by LPS. All results are shown as means ± SDs of 5 different experiments. *P < .05 and **P < .01 compared with HMGB1.