c-Src is activated in a MerTK-dependent manner upon AC treatment of DCs. (A-B) MerTK^+/+ or MerTK^−/− sDCs were incubated with ACs for the indicated times, and pc-Src and c-Src protein was measured by Western blot. Fold induction of pc-Src was determined by normalizing densitometric readings against control cultures. (C) MerTK^+/+ BMDCs were treated with ACs, c-Src was immunoprecipitated from whole-cell lysates, and in vitro c-Src kinase activity and c-Src protein were measured by Western blot. (D) MerTK^+/+ BMDCs were incubated with ACs for the indicated times, MerTK was immunoprecipitated from whole-cell lysates, and c-Src and MerTK protein were determined by Western blot. (E) MerTK^+/+ BMDCs were pretreated with αMerTK or isotype Ab for 1 hour, and then incubated with ACs. Whole-cell lysates were examined via Western blot for pSTAT3, pc-Src, and β actin. Data are representative of a minimum of 3 experiments.