c-Src activation is an upstream event of PI3K and STAT3 activation. MerTK^+/+ BMDCs were pretreated with E804 (10 nM) for 1 hour and incubated with ACs for indicated times. Whole-cell lysates were prepared to detect p-AKT, AKT, p-STAT3, and STAT3 via Western blot (A-B) or MerTK was immunoprecipitated with MerTK Ab and Western blots were probed for STAT3, PI3Kp85, p-Tyr, and MerTK (C-D).