CD56⁺ DC-like cells are CCR2⁺CD16⁻ consistent with the phenotype of inflammatory DC-like cells. CD56⁺ PBMCs or CD14⁺CD56⁻ PBMCs were isolated using either CD56 or CD14 microbeads. Cells were stained for the markers indicated and selective forward scatter/side scatter gating was used to analyze either monocytes/DC-like cells or lymphocytes. A representative donor is shown.