TSLP induces polarization of the DC cytoskeleton. (A) DCs on poly-lysine–coated coverslips were cultured in medium (untreated), influenza virus (Flu), LPS, or TSLP. Cells were stained for actin (red) and DAPI (blue) and observed under a fluorescence microscopy. Podosomes appeared as round actin-stained formations. Actin skeleton was reorganized in a polarized manner in TSLP-DCs. Data are from one representative of 5 independent experiments. (B) After 24 hours of culture, DCs with a polarized actin skeleton were quantified. Results are represented as percentage of total DCs. Data are mean ± SD; n = 5. *P < .05 vs untreated. ⁺P < .05 vs TSLP. (C) After 24 hours of culture, DCs were stained with an anti–α-tubulin mAb (green) and DAPI (blue). TSLP-DCs showed a reorganization of the microtubules. Data are from 1 representative of 5 independent experiments.