Myosin II is required for TSLP-induced polarization and motility. (A) After 24 hours of culture in the absence or presence of blebbistatin (50μM), TSLP-DCs were stained for myosin II (green), actin (red), and DAPI (blue). Blebbistatin inhibited TSLP-induced cytoskeleton rearrangements with a loss of cell polarization. Data are from 1 representative of 3 independent experiments. (B) DCs were cultured as described in Figure 1A, in the absence or presence of blebbistatin. An increased migration (6 hours) was observed after TSLP pretreatment, which was significantly decreased in the presence of blebbistatin (50μM). Each dot represents cell counts of individual experiments and bars represent the mean. *P < .05.