IL-12, but not IFN-α, regulates effector gene expression in human CD8⁺ T cells. (A) Differentially regulated genes by IL-12, IFN-α, and IL-12 + IFN-α were analyzed by Venn diagram to identify any genes commonly regulated by the different cytokine conditions. Individual segments are numbered corresponding to the gene list in supplemental Table 1. (B) Genes up-regulated by at least 1.7-fold were used to determine the Gene Ontology (GO) of the biological processes. The number of genes within a specific GO term is used as an indication of the magnitude of their functional correlation. (C) Normalized expression values of selected genes representing effector molecules are plotted as box-whisker bars (whiskers represent range).